Contact an Account ManagerAnthranoids in senna based drugs
Summary
Type: HPLC
Application #: 102630
Catalog#: 720013.40 - MN HPLC Column, Nucleosil C8, 100A, 5um, 4x250mm
Detection: photodiode array detector: dianthrones 270 nm; anthraquinones 435 nm
Conditions: eluent A: 0.02 M KH2PO4 solution, adjusted to pH 2.0 with o-phosphoric acid (85%),
eluent B: acetonitrile
injection: 20 μl.
gradient: 10 min 14% B, flow rate 1 ml/min, in 5 min to 20% B, 10 min at 20% B, in 1 min to 21% B, flow rate 0.8 ml/min, in 29 min to 80%B, in to min to to 14% B, flow rate 1 ml/min, then 13 min at 14% B
temperature 20 °C
Authors: Kabelitz L
Source: Dtsch Apotheker Ztg 134 (1994) 37 - 40
Details
Legend: Fig. 1: HPLC chromatogram of an anthranoid pool at 270 nm:
1. aloe emodin-8-glucoside
2. rhein-8-glucoside
3: sennoside D1
4: sennoside D
5: sennoside B
6: sennoside A1
7: sennoside C
8: senno